ReLASV® Pan-Lassa Combo NP/ Prefusion GP IgG/IgM ELISA Kit – RUO (10171)

$1,600.00$8,000.00

Designation: Semi-quantitative detection of anti-LASV nucleoprotein (NP) and Prefusion glycoprotein (GP) human IgG and IgM antibodies
Clade specificity: Pan-Lassa, antibodies to lineages II, III, IV nucleoprotein (NP) and glycoprotein (GP) antigens
Applications: ELISA microplate
Storage: 2-8°C
Shipping: Refrigerated; blue ice packs

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Description

This assay is a direct ELISA detecting Human IgG and/or IgM antibody specific for LASV NP and/or Prefusion GP. Diluted samples, Calibrator, and Negative Control are incubated in microwells coated with a mixture of recombinant LASV NP and Prefusion GP antigens. Incubation allows the anti-LASV antibodies present in the samples to react with the immobilized antigen mixture. After the removal of unbound serum or plasma proteins by washing, anti-human IgG (or IgM) antibodies, labeled with horseradish peroxidase (HRP), are added forming complexes with the bound IgG (or IgM) anti-LASV antibody. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of TMB substrate. Color develops in the wells at an intensity proportional to the concentration of IgG (or IgM) anti-LASV antibody in the sample.

Package insert for Pan-Lassa Combo NP-PfGP GM ELISA Kit (RUO) – pdf

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