The ReEBOV® Antigen Rapid Test is performed as a dipstick immunoassay. Sample consisting of serum, plasma or whole blood (finger stick or venous collection) is added to the Sample Pad. Inserting the dipstick into a test tube containing the Sample Buffer initiates the flow of sample through the reagent pads and across the nitrocellulose membrane. The EBOV VP40 specific antibody is striped onto nitrocellulose membrane in order to capture EBOV VP40 antigen. The EBOV VP40 specific antibody is also conjugated to gold nanoparticles and deposited in one of the rapid test reagent pads. As the assay develops, the EBOV VP40 antigen present in the sample forms immune-complexes with the anti-EBOV VP40 antibody – gold nanoparticles. As the VP40 antigen bound gold nanoparticles are captured by Test Line, the deposition of the gold nanoparticles generates a pink to red signal which corresponds to the concentration of EBOV VP40 antigen present in the sample. Excess gold nanoparticles are captured by the Control Line, indicating a valid result. Visual interpretation is made between 15 to 25 minutes signal development time.