News & Publications

Zalgen is committed to collaborate with our VHFC colleagues and others to advance the knowledge base of Viral Hemorrhagic Fevers and other viral diseases. Read the latest on how our work is making a difference.

Latest News

February 4, 2021 After a year of COVID-19, what can Maryland’s life sciences community learn from its collaborative response? — Technical.ly Baltimore 

December 22, 2020 Md. Life Sciences launches campaign to attract talent, investors — The Daily Record  

May 20, 2020 County awards grants to manufacture medical protective equipment — Bethesda Magazine

May 4, 2020 Maryland plays an outsized role in worldwide hunt for a coronavirus vaccine — The Washington Post

April 1, 2020 7 BioHealth Capital Region Diagnostics Companies Leading the Fight Against COVID-19 — BioBuzz

March 27, 2020 These Md. companies are racing to create coronavirus testing supplies — Baltimore Business Journal

March 25, 2020 How would we know if COVID-19 came from a lab? — SciWorthy

March 20, 2020 More than a dozen Md. biotech companies are working on new COVID-19 vaccines, tests — Baltimore Business Journal

March 20, 2020 These 17 Maryland biotech companies are collaborating in the fight against COVID-19 — Technical.ly Baltimore

March 9, 2020 ‘Coronaviruses are here to stay’: This Maryland biotech is developing tests for next time — Washington Business Journal

July 11, 2017 Tulane University awarded $12 million to create Lassa vaccine and treatment — Tulane News

June 1, 2017 Research team unravels elusive structure of Lassa virus, revealing vaccine target — Tulane News

September 5, 2017 New drug protects against advanced Lassa fever — Tulane News

March 17, 2017 Trump’s proposed NIH cuts could affect billions in D.C.-area research dollars — Washington Business Journal

February 12, 2016 Biotech industry: Stable funding, coordinated strategy needed to fight biological threats — The Business Journals

February 27, 2015 Zalgen Labs Co-founder Plans Return Work Trip To Africa (Video) — MyMCM Montgomery Community Media

February 24, 2015 Zalgen Labs Founder On Rapid Ebola Test (Video) — MyMCM Montgomery Community Media

Shaffer JG, Grant DS, Schieffelin JS, et.al. (2021) Space-Time Trends in Lassa Fever in Sierra Leone by ELISA Serostatus, 2012–2019. Microorganisms. 2021 Mar 12;9(3):586. Kenema Government Hospital (KGH) maintains one of only a few Lassa Fever (LF) isolation facilities in the world with year-round diagnostic testing. The study focused on space-time trends for LF occurring in Sierra Leone between 2012 and 2019 to provide a current account of LF in the wake of the 2014–2016 Ebola epidemic. Data were analyzed for 3277 suspected LF cases and classified as acute, recent, and non-LF or prior LF exposure using Zalgen Labs ReLASV ELISA kits. Presentation rates for acute, recent, and non-LF or prior LF exposure were 6.0% (195/3277), 25.6% (838/3277), and 68.4% (2244/3277), respectively. Among 2051 non-LF or prior LF exposures, 33.2% (682/2051) tested positive for convalescent LF exposure. The overall LF case- fatality rate (CFR) was 78.5% (106/135). The findings suggest that LF remains endemic in Sierra Leone and that caseloads are likely to resume at levels observed prior to the Ebola epidemic. The results provide insight on the current epidemiological profile of LF in Sierra Leone to facilitate LF vaccine studies and accentuate the need for LF cohort studies and continued advancements in LF diagnostics.

Heinrich ML, Boisen ML, Nelson DKS, et.al. Antibodies from Sierra Leonean and Nigerian Lassa fever survivors cross‑react with recombinant proteins representing Lassa viruses of divergent lineages. Scientific Reports 10:16030 (2020). As part of CEPI ’s (Coalition for Epidemic Preparedness Innovations) Lassa vaccine development program, we assessed the potential of the human immune system to mount cross-reactive and cross-protective humoral immune responses to antigens from the most prevalent LASV lineages (lineages II and III in Nigeria and lineage IV in Sierra Leone). IgG and IgM present in the blood of Lassa fever survivors from Nigeria or Sierra Leone exhibited substantial cross-reactivity for binding to LASV nucleoprotein and two engineered (linked and prefusion) versions of the glycoproteins (GP) of lineages II–IV. These studies provide guidance for comparison of humoral immunity to LASV of distinct lineages following natural infection or immunization.

Cross RW, Agans KN, Prasad AN, et.al. Intranasal exposure of African green monkeys to SARS-CoV-2 results in acute phase pneumonia with shedding and lung injury still present in the early convalescence phase. Virology Journal 17: 125 (2020) Follow up to recently reported development of the first African green monkey (AGM) model for COVID-19 based on a combined liquid intranasal (i.n.) and intratracheal (i.t.) exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Assessment of an i.n. particle only route of exposure using the LMA mucosal atomization device (MAD). Findings are consistent with human COVID-19 further demonstrating that the AGM faithfully reproduces the human condition. Study utilized Zalgen Labs ReSARS CoV-2 Nucleoprotein ELISA kits to measure specific IgG antibodies in AGM sera.

Barnes KG, Lachenauer AE, Nitido A, et.al. Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time. Nature Communications 11: 4131 (2020). Development of a CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. Assessment on laboratory and clinical samples assay sensitivity and capacity to handle virus-specific diagnostic challenges, and safety testing to demonstrate the efficacy of the HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extraction. Development of a user-friendly protocol and mobile application (HandLens) to report results, facilitating SHERLOCK’s use in endemic regions.

Boisen, M.L., Uyigue, E., Aiyepada, J. et al. Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreakSci Rep 10, 8724 (2020). Evaluation of the Zalgen ReLASV Pan-Lassa Antigen Rapid Test, a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). The performance of the Pan-Lassa RDT was compared to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.

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